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Merck

HPLC Analysis of Methylphenidate (Ritalin) Enantiomers on Astecยฎ CHIROBIOTICยฎ V2

HPLC Analysis of Methylphenidate (Ritalin) Enantiomers on Astec® CHIROBIOTIC® V2 application for HPLC

Materials

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15024AST

Astecยฎ CHIROBIOTICยฎ V2 Chiral HPLC Column

5 μm particle size, L ร— I.D. 25 cm ร— 4.6 mm

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M2892

Methylphenidate hydrochloride

CONDITIONS

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

mobile phase

15 mM ammonium acetate in methanol

flow rate

1 mL/min

column temp.

25 ยฐC

detector

UV, 215 nm

injection

5 μL

sample

1 mg/mL in methanol

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Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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