HPLC Analysis of Glutamic Acid Enantiomers on Astecยฎ CHIROBIOTICยฎ T (25 ยฐC)
Materials
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Astecยฎ CHIROBIOTICยฎ T Chiral HPLC Guard Column
Cartridge, 5 μm particle size, L ร I.D. 2 cm ร 4 mm๋ถ์ ์ปฌ๋ผ
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L-Glutamic acid
from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%L-Glutamic acid
certified reference material, TraceCERTยฎ, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)
flow rate
1 mL/min
column temp.
25 ยฐC
detector
UV, 205 nm
injection
10 μL
sample
300 μg /mL in 30:70 water:methanol
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Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC