HPLC Analysis of Fusion Protein on Zenix^®-C SEC-300, Effect of Mobile Phase Composition

column

Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)

gradient

(A) lower, 150 mM sodium phosphate buffer, pH 7.0 (B) middle, 150 mM sodium phosphate buffer, pH 7.0 + 10% acetonitrile (C) top, 150 mM sodium phosphate buffer, pH 7.0 + 20 mM NaCl

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 214 nm

injection

10 μL

sample

1 mg/mL fusion protein, MW 170 kD, pl 6.8-7.0