HPLC Analysis of Cosmetic Preservatives on Ascentisยฎ Express RP-Amide
Materials
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Ascentisยฎ Express RP-Amide, 2.7 ฮผm HPLC Column
2.7 μm particle size, L ร I.D. 10 cm ร 3 mmํ์ค ๋ฌผ์ง
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CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 μm particles (53918-U)
mobile phase
[A] water; [B] methanol; (60:40, A:B)
flow rate
0.6 mL/min
pressure
2900 psi (200 bar)
column temp.
50 ยฐC
detector
UV, 250 nm
injection
1 μL
sample
methyl-, benzylparaben (50 mg/L), ethylparaben (30 mg/L), propyl-, butylparaben (100 mg/L), 2-phenoxyethanol (1.5 g/L),1-phenoxy-2-propanol (3 g/L), all in 40% methanol
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๋ถ์ ๋ฉ๋ชจ
With the publication Borremans, M. et.al. 2004, Chromatographia 59: 47-53 as a reference, this method is 10% faster, but with superior resolution of critical pairs; in this case, the minimum USP resolution is 2.4.
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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany