Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)

Evgeni Eltzov, Avital Cohen, Robert S Marks

초록

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS

제품 번호

브랜드

제품 설명

227056

Sigma-Aldrich

N,N-Dimethylformamide, anhydrous, 99.8%

494488

Sigma-Aldrich

N,N-Dimethylformamide, biotech. grade, ≥99.9%

D158550

Sigma-Aldrich

N,N-Dimethylformamide, ReagentPlus^®, ≥99%

411027

Sigma-Aldrich

Piperidine, ≥99.5%, purified by redistillation

571261

Sigma-Aldrich

Piperidine, biotech. grade, ≥99.5%

47608

Sigma-Aldrich

Formaldehyde solution, BioReagent, ≥36.0% in H₂O (T)

F8775

Sigma-Aldrich

Formaldehyde solution, 36.5-38% in H₂O

32160

Sigma-Aldrich

Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless

33120

Sigma-Aldrich

N,N-Dimethylformamide, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)

378860

Sigma-Aldrich

Sodium chloride, random crystals, optical grade, 99.9% trace metals basis

346136

Sigma-Aldrich

Diethyl ether, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor

309966

Sigma-Aldrich

Diethyl ether, suitable for HPLC, ≥99.9%, inhibitor-free

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

71376

Sigma-Aldrich

Sodium chloride, BioUltra, ≥99.5% (AT)

178810

Sigma-Aldrich

Tetrahydrofuran, ≥99.0%, contains 200-400 ppm BHT as inhibitor, ReagentPlus^®

401757

Sigma-Aldrich

Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free

186562

Sigma-Aldrich

Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%

87368

Sigma-Aldrich

Tetrahydrofuran, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%

24216

Sigma-Aldrich

Chloroform, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)

47083-U

Supelco

Formaldehyde solution, stabilized with methanol, ~37 wt. % in H₂O, certified reference material

32203

Sigma-Aldrich

Diethyl ether, contains BHT as inhibitor, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

534064

Sigma-Aldrich

Acetone, histological grade, ≥99.5%

179124

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

293245

Sigma-Aldrich

1-Octanol, suitable for HPLC, ≥99%

179973

Sigma-Aldrich

Acetone, Laboratory Reagent, ≥99.5%

320110

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

24201

Sigma-Aldrich

Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)

24004

Sigma-Aldrich

Diethyl ether, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)

673811

Sigma-Aldrich

Diethyl ether, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor

496189

Sigma-Aldrich

Chloroform, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer