추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
2F10, monoclonal
recombinant monoclonal
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt ~97.68 kDa
observed mol wt ~97 kDa
종 반응성
human, mouse
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 2F10 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects F4/80 (Adhesion G protein-coupled receptor E1). It targets an epitope within 15 amino acids from the N-terminal, extracellular domain.
면역원
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human F4/80 (Adhesion G protein-coupled receptor E1).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected F4/80 in Jurkat cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected F4/80 in Raji, NIH3T3, and human spleen tissue lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected F4/80 in Jurkat cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected F4/80 in one million Jurkat cells.
Affinity Binding Assay: A representative lot of this antibody bound F4/80 peptide with a KD of 2.0 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: 1:100 from a representative lot detected F4/80 in human spleen and human bone marrow tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected F4/80 in Jurkat cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected F4/80 in Raji, NIH3T3, and human spleen tissue lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected F4/80 in Jurkat cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected F4/80 in one million Jurkat cells.
Affinity Binding Assay: A representative lot of this antibody bound F4/80 peptide with a KD of 2.0 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: 1:100 from a representative lot detected F4/80 in human spleen and human bone marrow tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-F4/80, clone 2F10 ZooMAb®, Cat. No. ZRB1277, is a recombinant Rabbit monoclonal antibody that targets F4/80 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
표적 설명
Adhesion G protein-coupled receptor E1 (UniProt: Q14246; also known as EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, F4/80) is encoded by the ADGRE1 (also known as EMR1, TM7LN3) gene (Gene ID: 2015) in human. F4/80 is multi-pass membrane glycoprotein of the G-protein coupled receptor 2 family that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It also has six EGF-like domains in the N-terminal region followed by a serine/threonine-rich stalk region. F4/80 is involved in cell adhesion and in cell-cell interactions involving the immune system. F4/80 is reported to be a pan-macrophage marker mouse to detect the distribution and localization of tissue macrophages and to examine the phenotypic heterogeneity and activation state of resident macrophages. Although not required for normal macrophage development in murine species, F4/80 is shown to be essential for the induction of efferent CD8+ regulatory T-cells, which is needed for peripheral tolerance. In human F4/80 expression is restricted to eosinophils. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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