추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
3I14, recombinant monoclonal
monoclonal
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt ~56.08 kDa
observed mol wt ~56 kDa
종 반응성
rat, mouse, human
종 반응성(상동성에 의해 예측)
canine
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 3I14 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Chromobox protein homolog 2 (CBX2). It targets an epitope within 25 amino acids from the N-terminal region.
면역원
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human Chromobox protein homolog 2 (CBX2).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CBX2 in HEK293 cell nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CBX2 in nuclear extract from NIH3T3 cells, HeLa cells, and L6 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CBX2 in HeLa, A431, HepG2, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CBX2 in human skin tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CBX2 peptide with a KD of 8.7 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HEK293 cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CBX2 in HEK293 cell nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CBX2 in nuclear extract from NIH3T3 cells, HeLa cells, and L6 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CBX2 in HeLa, A431, HepG2, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CBX2 in human skin tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CBX2 peptide with a KD of 8.7 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CBX2, clone 3I14 ZooMAb®, Cat. No. ZRB1084, is a recombinant Rabbit monoclonal antibody that specifically targets CBX2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
표적 설명
Chromobox protein homolog 2 (UniProt: Q14781; also known as CBX2) is encoded by the CBX gene (Gene ID: 84733) in human. CBX2 is a component of a Polycomb group (PcG) multiprotein PRC1-like complex that is expressed during interphase and metaphase. It plays a role in the lineage differentiation of the germ layers in embryonic development and is involved in sexual development, acting as activator of NR5A1 expression. It is shown to be localized in distinct foci on chromatin and in chromocenters. CBX2 is reported to be expressed from week 9 to week 25 post-fertilization in both male and female gonads. It is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in murine models is shown to result in male-to-female gonadal sex reversal. Levels of CBX2 are reported to decrease in macrophages upon viral infection and knockdown of CBX2 gene in macrophages can inhibit virus-induced production of interferon-. CBX2 is reported to bind and recruit Lysine demethylase 6B (Jmjd3) to the IFN- promoter, leading to demethylation of H3K27me3 and increased transcription of IFN-β. Mutations in CBX2 gene are associated with gonadal dysgenesis in humans. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sun, D., et al. (2019). Protein Cell 10(4); 285-294).
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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