추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
5C15, recombinant monoclonal
monoclonal
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt ~431.76 kDa
종 반응성
human
종 반응성(상동성에 의해 예측)
monkey
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 5C15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Histone-lysine N-methyltransferase 2A (MLL/HRX). It targets an epitope within 21 amino acids from the MLL cleavage product C180 region.
면역원
KLH-conjugated linear peptide corresponding to 21 amino acids from the p180 C-terminal subunit of human Histone-lysine N-methyltransferase 2A (MLL/HRX).
애플리케이션
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MLL/HRX in human kidney tissue sections.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound MLL peptides with a KD of 1.2 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from representative lot detected MLL/HRX CT in HeLa and A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected MLL/HRX in human kidney tissue sections.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound MLL peptides with a KD of 1.2 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from representative lot detected MLL/HRX CT in HeLa and A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MLL/HRX CT, clone 5C15 ZooMAb®, Cat. No. ZRB1061, is a recombinant Rabbit monoclonal antibody that specifically targets MLL/HRX and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Immunohistochemistry (Paraffin).
표적 설명
Histone-lysine N-methyltransferase 2A (UniProt: Q03164; also known as EC:2.1.1.354, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, MLL, Trithorax-like protein, Zinc finger protein HRX) is encoded by the KMT2A (also known as ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1) gene (Gene ID: 4297) in human. MLL is a histone methyltransferase that plays an essential role in early development and hematopoiesis. It is expressed in heart, lung, brain, and in T and B lymphocytes. Catalytic subunit of the MLL complex mediates both methylation of lysine 4 of histone H3 (H3K4me) complex and acetylation of lysine 16 of histone H4 (H4K16ac). By itself it has weak methyltransferase activity and requires another component of the MLL complex to obtain full methyltransferase activity. MLL does not display any activity towards histone H3 phosphorylated on threonine 3 and has less activity towards histone H3 dimethylated on arginine 8 and lysine 9. However, it displays high activity towards histone H3 acetylated on lysine 9. MLL is normally cleaved into a N-terminal p320 and a C-terminal p180 cleavage product. This cleavage is brought about by taspase 1 (TASP1) at cleavage site 1 (aa 2666-2667) and cleavage site 2 (aa 2718-2719). The p320 and p180 units can heterodimerize via SET and FYRC domains. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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