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Merck
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주요 문서

Z734438

ThermalSeal RTS Sealing Films

for qPCR, storage & crystallization, non-sterile

동의어(들):

qPCR plate seal, storage plate seal

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About This Item

UNSPSC 코드:
41122410
NACRES:
NB.22

material

polyolefin film
silicone/adhesive

무균

non-sterile

포장

pack of 100 ea

제조업체/상표

Excel Scientific TSS-RTQ-100

기술

PCR: suitable
qPCR: suitable

길이 × 너비

76.2 mm × 133.4 mm

너비 × 길이

7.62 cm × 13.34 cm

색상

clear

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일반 설명

  • High optical clarity
  • Minimal to no autofluorescence
  • Chemically inert; no extractables except at extreme pH
  • DMSO resistant for HTS
  • Heat resistant, recommended for temperatures from
  • -70°C to +100°C
  • Certified DNase, RNase, and Nucleic Acid free
  • Fit within raised plate rim to prevent loss of seal due to film lifting
  • Silicone adhesive forms the strongest available seal for evaporation prevention
  • Non-tacky adhesive layer simplifies handling of film prior to sealing
ThermalSeal RTS sealing film is a 50 μm thick polyolefin with 50 μm inert encapsulated silicone adhesive. They are optically clear, with low autofluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert.

ThermalSeal RTS films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.

Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.

  • High optical clarity
  • Minimal to no autofluorescence
  • Chemically inert; no extractables except at extreme pH
  • DMSO resistant for HTS
  • Heat resistant, recommended for temperatures from
  • -70°C to +100°C
  • Certified DNase, RNase, and Nucleic Acid free
  • Fit within raised plate rim to prevent loss of seal due to film lifting
  • Silicone adhesive forms the strongest available seal for evaporation prevention
  • Non-tacky adhesive layer simplifies handling of film prior to sealing

법적 정보

ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RTS is a trademark of Excel Scientific, Inc.

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

프로토콜

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt® ReadyMix™.

Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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