Name: Trypan Blue solution
Brand: SIGMA

로그인조직 및 계약 가격 보기

About This Item

실험식(Hill 표기법):

C₃₄H₂₄N₆Na₄O₁₄S₄

CAS Number:

72-57-1

Molecular Weight:

960.81

Beilstein:

4360496

MDL number:

MFCD00003969

UNSPSC 코드:

12352207

PubChem Substance ID:

57654704

NACRES:

NA.75

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

무균

sterile-filtered

Quality Level

200

양식

liquid

저장 조건

dry at room temperature

농도

0.4%

기술

cell culture | mammalian: suitable
tissue processing: suitable

응용 분야

cell analysis

배송 상태

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

For the same product in a convenient lyophilized (powder) format, please see T6146

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

애플리케이션

Trypan Blue solution has been used:

in cell viability assay to count viable and dead cells
in rescue assay to count viable cells
in trypan blue exclusion counting method to determine proliferation curve
as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
to count GEC cells using a hemocytometer