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Merck
모든 사진(1)

주요 문서

SRP5232

Sigma-Aldrich

EIF2S1, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

동의어(들):

EIF-2, EIF-2α, EIF-2A, EIF2, EIF2A

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About This Item

CAS Number:
UNSPSC 코드:
12352200
NACRES:
NA.32

생물학적 소스

human

재조합

expressed in E. coli

분석

≥70% (SDS-PAGE)

양식

buffered aqueous glycerol solution

분자량

~40 kDa

NCBI 수납 번호

응용 분야

cell analysis

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... EIF2S1(1965)

일반 설명

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) is a 35kDa protein. It is the α subunit of the eukaryotic initiation factor 2 (eIF2α). The gene encoding it is localized on human chromosome 14q23.3.

생화학적/생리학적 작용

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) catalyzes the first regulated step of protein synthesis initiation and promotes the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. The Ser51 in the mature human EIF2-α protein acts as the sole site of phosphorylation that leads to repression of protein synthesis. EIF2-α also plays an essential role in erythropoiesis.

물리적 형태

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

제조 메모

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

픽토그램

Health hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

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문서 라이브러리 방문

Direct interaction between STAT3 and EIF2AK2 controls fatty acid-induced autophagy
Mireia N
Autophagy (2012)
V K Pathak et al.
Molecular and cellular biology, 8(2), 993-995 (1988-02-01)
The phosphorylation of the alpha-subunit of initiation factor eIF-2 leads to an inhibition of protein synthesis in mammalian cells. We have performed site-directed mutagenesis on a cDNA encoding the alpha-subunit of human eIF-2 and have replaced the candidate sites of
A SNP microarray and FISH-based procedure to detect allelic imbalances in multiple myeloma: An integrated genomics approach reveals a wide gene dosage effect
Luca A
Genes (2009)
Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2a) dephosphorylation in mammalian development
Heather P
Proceedings of the National Academy of Sciences of the USA (2008)
ER stress and autophagy contribute to CsA-induced death of malignant glioma cells
Iwona Anna
Autophagy (2012)

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