SRP0243

aRAF Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• 동의어(들): A-Raf proto-oncogene, PKS2, v-Raf murine sarcoma 3611 viral oncogene homolog
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥80% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 63.6 kDa
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: >0.02 mg/mL
• NCBI 수납 번호: NM_001654
• UniProt 수납 번호: P10398
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... ARAF(369)

설명

일반 설명

Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.