SRE0119

dsDNase

For gDNA removal, recombinant, expressed in Pichia pastoris, Heat labile, Triton-free

• 동의어(들): HL-dsDNase, heat labile double strand specific deoxyribonuclease
• UNSPSC 코드: 12352204
• NACRES: NA.77

속성

• 재조합: expressed in Pichia pastoris
• Quality Level: 200
• 구성: Triton-free
• 포장: pkg of 250 U
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Double-strand specific DNases (dsDNases) are unique double-strand specific endonucleases. As they do not digest ssDNA or RNA, they can be used to specifically remove dsDNA in the presence of other nucleic acids.

애플리케이션

This heat labile dsDNase removes genomic DNA from RNA preparations with minimal impact on RNA integrity.with minimal impact on RNA integrity

특징 및 장점

Due to the mild inactivation temperature required, this dsDNase can remove genomic DNA from RNA perparations and have minimal impact onto RNA quality and quantity.

단위 정의

One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl₂.

재구성

In solution

기타 정보

The heat labile dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable