SAE0219

Mucin Degradation Enzyme

for preparative digestions, powder

• UNSPSC 코드: 41121800

속성

• Quality Level: 200
• 양식: powder
• 유통기한: 2 yr (retest)
• 저장 조건: protect from light
• 배송 상태: dry ice

설명

일반 설명

Mucin Degradation Enzyme is a metalloprotease useful for digesting heavily O-glycosylated mucin proteins. This product is made for preparative scale digestions of viscous material that is dense with mucin-type glycoproteins.

애플리케이션

For use as a sample preparation tool in research and discovery. Used for proteolytic degradation of mucin-dense samples to allow for downstream processing.

생화학적/생리학적 작용

The Mucin Degradation is based on a bacterial protease that specifically recognizes densely glycosylated mucins and mucin-domains, and proteolytically cleaves the peptide backbone. In nature this presumably enhances the virulence of the pathogenic microbe, but in the laboratory is a useful way of accessing molecular structures in tissues normally shielded by mucins.

특징 및 장점

• A promiscuous Mucin specific protease.
• Tool for viscosity reduction and tissue breakdown of mucin containing samples.
• Not inhibited by most general protease inhibitors such as AEBSF, Aprotinin, Bestatin, E-64, Pepstatin and Leupeptine. When needed digests can be done in presence of the ReadyShield^® Protease Inhibitor Cocktail PIC0002.
• Mucin Degradation Enzyme can be used to liberate soluble mucin fragments from cells or tissue without mechanical disruption or reduction.
• Mucin Degradation Enzyme can be used for enzymatic hydrolysis of mucins in the isolation of glyco-RNA samples.

물리적 형태

Product is supplied as lyophilized powder containing the indicated amount of protein.

제조 메모

Solution of Mucin Degradation Enzyme can be prepared by reconstitution of the lyophilized powder at a concentration of 1-5mg/ml protein. See CoA for lot specific percent protein in powder. The reconstituted protein solution may be clear or hazy and is stable for 2 weeks at 2-8 °C. For longer storage, aliquot the protein solution and store at -20 °C. The enzyme solution should be protected from direct light.

저장 및 안정성

The product retains activity for at least 2 years when stored lyophilized at -20°C.

기타 정보

For applications that require more precision with highly purified enzyme like glycoproteomic experiments please consider using Mucinase StcE (SAE0202) and Mucinase SmE (SAE0220).

법적 정보

Under licensing from Stanford University.

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable