SAB5900208

Alpaca Anti-mCherry, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• 동의어(들): MCherry fluorescent protein
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: alpaca
• Quality Level: 100
• 재조합: expressed in E. coli
• 결합: unconjugated
• 항체 형태: affinity purified VHH antibody
• 클론: RP55, monoclonal
• 분석: >95% (SDS-PAGE)
• 양식: lyophilized solid
• 분자량: 14.3 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• 정제법: immobilized metal affinity chromatography (IMAC)
• 농도: 1 mg/mL
• 기술: ELISA: 1:2000-1:10000; immunofluorescence: 1:500-1:2000; western blot: 1:1000-1:5000
• 색상: colorless
• pH: ( in PBS, pH 7.4)
• 동형: VHH
• 단백질 ID 수납 번호: UFQ89828.1
• UniProt 수납 번호: A0A4D6FVK6
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel. Alpaca derived anti-mCherry VHH single domain antibody (molecular weight: 14.3 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

특이성

mCherry

면역원

Recombinant full length mCherry protein

애플리케이션

ELISA, WB, IF

포장

50 µg per vial / 100 µg per vial

물리적 형태

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.11

재구성

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

저장 및 안정성

Stable for 1 year at 4°C from date of receipt.

기타 정보

Storage at 4°C; Do not freeze.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable