SAB5200017

Monoclonal Anti-HIF-1 α antibody produced in mouse

clone ESEE122, 1 mg/mL, purified immunoglobulin

• 동의어(들): Anti-ARNT interacting protein, Anti-HIF1 alpha, ESEE122, Anti-HIF1A, Anti-Hypoxia inducible factor 1 alpha, Anti-MOP1, Anti-PASD8
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: ESEE122, monoclonal
• 양식: buffered aqueous glycerol solution
• 종 반응성: bovine, mouse, human, rat
• 농도: 1 mg/mL
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_034561.2
• UniProt 수납 번호: Q16665
• 배송 상태: wet ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Hif1a(15251)

설명

일반 설명

Hypoxia-inducible factor-1 α (HIF1 α) gene encodes the α subunit of HIF-1. HIF-1α contains oxygen-dependent degradation (ODD) domain that mediates the interaction between HIF-1α and the E3 ubiquitin ligase complex, which in turn facilitates continuous poly ubiquitination of HIF-1α in normoxic cells. The gene encoding it is localized on human chromosome 14q23.2. HIF1 α belongs to the basic helix-loop-helix family of transcription factors.

특이성

Specific for HIF1-alpha.

면역원

Recombinant fragment corresponding to amino acids 329-530

애플리케이션

Monoclonal Anti-HIF-1 α antibody produced in mouse has been used in siRNA assay and western blot analysis.

생화학적/생리학적 작용

Hypoxia-inducible factor-1(HIF1) plays an essential role in cellular and systemic homeostatic responses to hypoxia. During hypoxia, the two subunits of this factor undergo post-translational modifications which in turn promote transactivation. HIF-1α gene loss is associated with development of nonpapillary renal carcinomas. The encoded protein functions as a key regulator of the vascular endothelial growth factor (VEGF) under hypoxic response in human stromal cells in vitro. HIF-1α protein expression is often seen in invasive breast cancer. Experimental data demonstrates that downregulation of HIF-1α decreases tumorigenicity of MCF-7 (human breast adenocarcinoma cell line) cells and propose a promising combination of both anti-HIF-1 strategy and traditional chemotherapy to improve cancer treatment.

특징 및 장점

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

물리적 형태

Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable