SAB4200053

Anti-DNA polymerase δ p125 antibody, Rat monoclonal

clone PDG 5G1, purified from hybridoma cell culture

• 동의어(들): Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 200
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: PDG 5G1, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~125 kDa
• 종 반응성: human, mouse, rat
• 포장: antibody small pack of 25 μL
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using Jurkat cells extract
• UniProt 수납 번호: P28340
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... POLD1(5424)

설명

일반 설명

Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.

면역원

human p125

애플리케이션

Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• co-immunoprecipitation

생화학적/생리학적 작용

The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.

물리적 형태

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable