S8695

Anti-Supervillin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Supervillin Isoform 1
• MDL number: MFCD08705523
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~250 kDa
• 종 반응성: human, mouse
• 기술: indirect immunofluorescence: 10-20 μg/mL using human HeLa cells; western blot: 2.5-5 μg/mL using whole extracts of differentiated mouse C2 cells; western blot: 5-10 μg/mL using whole extracts of human HeLa cells
• UniProt 수납 번호: O95425
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SVIL(6840) ; mouse ... Svil(225115)

설명

일반 설명

Supervillin is an F-actin-binding protein originally isolated from bovine neutrophils. It has two isoforms of reported 205/250 kDa. The longer isoform named archvillin is muscle specific. Supervillin is a tightly bound peripheral membrane protein that is concentrated at sites of epithelial cell-cell adhesion. The COOH-terminus of supervillin is homologous to villin/gelsolin. The NH2-terminus contains functional nuclear localization sequences and F-actin and myosin II binding domains. Supervillin is found in many cells of several species, but is most abundant in muscle, bone marrow, thyroid gland and salivary gland.

면역원

synthetic peptide corresponding to amino acid residues 900-918 of human supervillin with C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by two amino acids in mouse.

애플리케이션

Anti-Supervillin antibody produced in rabbit has been used in:

• immunoblotting
• chromatin immunoprecipitation (ChIP)
• immunofluorescence

생화학적/생리학적 작용

Supervillin participates in cell-cell adhesion, motility regulation and information transfer between cell compartments. It has been suggested that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains. Supervillin has been identified as a transcriptional activator of the androgen receptor. Increased levels of supervillin are found in many carcinoma cell lines, including HeLa S3 cervical carcinoma, SW480 adenocarcinoma and A549 lung carcinoma cells.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)