S8321

Anti-Sonic Hedgehog antibody, Mouse monoclonal

clone SH154, purified from hybridoma cell culture

• 동의어(들): Anti-Shh
• MDL number: MFCD06798717
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: SH154, monoclonal
• 양식: buffered aqueous solution
• 종 반응성: human, rat, mouse
• 농도: ~2 mg/mL
• 기술: immunohistochemistry: 1:500 using formaldehyde-fixed human tissue sections; indirect ELISA: 2 μg/mL; western blot: 2 μg/mL using recombinant mouse SHH peptide
• 동형: IgG1
• UniProt 수납 번호: Q15465
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SHH(6469) ; mouse ... Shh(20423) ; rat ... Shh(29499)

설명

일반 설명

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog antibody recognizes human, rat and mouse Shh.

특이성

Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody recognizes human, rat, and mouse Shh.

애플리케이션

Anti-Sonic Hedgehog antibody may be used for immunoblotting at a working concentration of ~2 μg/ml. A working antibody concentration of ~2 μg/ml is recommended for ELISA. For immunohistochemistry a dilution of 1:500 may be used.

Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody may be used in ELISA, immunoblotting, and immunohistochemistry. It is used as a probe to determine the presence and roles of Sonic Hedgehog in processes such as patterning during embryogenesis.

생화학적/생리학적 작용

Sonic Hedgehog (Shh) is an important cell signaling molecule expressed during embryonic development. Shh is involved in patterning of the developing embryonic systems such as the nervous system, somites, and limbs. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened. Shh protein is expressed in key embryonic tissues such as the Hensen′s node, zone of polarizing activity in the posterior limb bud, notochord, and floor plate of the neural tube. Downstream targets of Shh include the transcription factors Gli3, responsible for Greigs polycephalosyndactyly in humans, and Hoxd13, responsible for polysyndactyly.

물리적 형태

Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)