추천 제품
제품명
Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)
Quality Level
설명
stability: stable to 0.2M NaOH
양식
suspension (20% aqueous ethanol)
특징
autoclavable
Matrix
crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
입자 크기
25-75 μm (wet)
공극 크기
10-2000 kDa MW range (dextrans)
20-8000 kDa MW range (globular proteins)
호환성
mode of use size exclusion chromatography
저장 온도
2-8°C
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관련 카테고리
일반 설명
- Supplied swollen, suspended in 20% aqueous ethanol
- Autoclavable
- Stable to 0.2N sodium hydroxide (NaOH)
- Store at refrigerated temperature (2-8 °C)
S400HR-750ML′s updated product is GE17-0609-01
애플리케이션
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.
법적 정보
Sephacryl is a registered trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point (°F)
100.4 - 109.4 °F - closed cup
Flash Point (°C)
38 - 43 °C - closed cup
개인 보호 장비
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Enhancement of drug delivery to bone: Characterization of human tissue-nonspecific alkaline phosphatase tagged with an acidic oligopeptide
Nishioka T, et al.
Molecular Genetics and Metabolism, 88(3), 244-255 (2006)
Michael J Lodes et al.
Methods in molecular medicine, 94, 91-106 (2004-02-13)
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects. Here
E Käslan et al.
The Journal of biological chemistry, 269(19), 14103-14110 (1994-05-13)
During purification of a strand exchange activity from Schizosaccharomyces pombe using the three-strand reaction of double-stranded linear and circular single-stranded DNA, we identified p190/210 as an activity that stimulated the strand exchange activity of p140exo2 by about 10-fold. The accompanying
Masaharu Tsuji et al.
International journal of systematic and evolutionary microbiology, 69(4), 944-948 (2019-01-04)
Four strains isolated from sediment sampled at the front of a retreating glacier on northern Ellesmere Island in the Canadian high Arctic, namely JCM 32575T, JCM 32576, JCM 32577 and JCM 32578, belong to a novel psychrophilic basidiomycetous yeast species
Sinu P John et al.
Journal of virology, 81(17), 8967-8976 (2007-06-15)
The Ebola virus (EBOV) genome encodes for several proteins that are necessary and sufficient for replication and transcription of the viral RNAs in vitro; NP, VP30, VP35, and L. VP30 acts in trans with an RNA secondary structure upstream of
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