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Merck
모든 사진(1)

주요 문서

R6148

Sigma-Aldrich

RNase A solution

동의어(들):

GenElute RNAse A

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About This Item

UNSPSC 코드:
12352204
NACRES:
NA.52

생물학적 소스

bovine pancreas

Quality Level

양식

liquid

특이 활성도

1500 units/mL
3000 units/mL

농도

20 mg/mL in Tris buffer

기술

DNA purification: suitable

저장 온도

room temp

유전자 정보

일반 설명

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute kits.

애플리케이션

RNase A solution has been used:

  • in apoptosis assay of Hep-2 cells
  • for the colorimetric quantification of methylated DNA
  • for DNA and RNA extraction
  • in DNA laddering analysis
  • in cell cycle analysis
  • as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

생화학적/생리학적 작용

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.

제조 메모

For usage requirements, please refer to the kit procedure.

법적 정보

GenElute is a trademark of Sigma-Aldrich Co. LLC

샘플 준비

제품 번호
설명
가격

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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