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Merck
모든 사진(2)

주요 문서

P3134

Sigma-Aldrich

Phosphodiesterase I from Crotalus adamanteus venom

Type VI, crude dried venom

동의어(들):

5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase

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About This Item

CAS Number:
EC 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

유형

Type VI

Quality Level

양식

crude dried venom

저장 온도

−20°C

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일반 설명

Research area: Cell signaling. PhosphodiesteraseI (PDEI) exists in three isoforms PDE1A, PDE1B, and PDE1C(2) are localized to the central nervous system, cardiovascular, and other organs.

애플리케이션

Phosphodiesterase I from Crotalus adamanteus venom has been used:
  • for cleaving the 3′-5′ internucleotide bonds in the linear chains of the RNA oligomers
  • as a model for the hydrolysis of prodrugs in phosphodiesterase assay
  • as a component in the nucleotide cleavage buffer

생화학적/생리학적 작용

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C. PhosphodiesteraseI (PDEI) can hydrolyze 3′,5′-cyclic adenosine monophosphate (cAMP) and3′,5′-cyclic guanosine monophosphate (cGMP). It binds to Ca2+/calmodulin(CaM) that lead to the stimulation of its catalytic activity.PDEIacts as a biotherapeutic for the treatment of cancer, heart diseases,pulmonary, metabolic, neurocognitive, renal, endocrine abnormalities, andneurological disorders.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

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문서 라이브러리 방문

Chemical toxicity testing in vitro using cytochrome P450--expressing cell lines, such as human CYP1B1
Landsiedel R, et al.
Nature Protocols, 6(5), 677-677 (2011)
Formation of RNA oligonucleotides over the mineral surface preliminary irradiated with UV light
Otroshchenko VA and Vasilyeva NV
Reaction Kinetics and Catalysis Letters, 97(1), 151-156 (2009)
Anatoly I Dragan et al.
Nucleic acids research, 32(17), 5192-5197 (2004-10-02)
The interaction of proteins with DNA results, in some cases, in DNA bending, and this might have functional importance. However, when the protein-induced bending of DNA is small, its measurement presents a problem. It is shown that the fluorescence resonance
Ester prodrugs of acyclic nucleoside thiophosphonates compared to phosphonates: synthesis, antiviral activity and decomposition study
Roux L, et al.
European Journal of Medicinal Chemistry, 63, 869-881 (2013)
Suxiang Chen et al.
Scientific reports, 9(1), 6078-6078 (2019-04-17)
Antisense oligonucleotide (AO)-mediated splice modulation has been established as a therapeutic approach for tackling genetic diseases. Recently, Exondys51, a drug that aims to correct splicing defects in the dystrophin gene was approved by the US Food and Drug Administration (FDA)

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