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Merck
모든 사진(2)

주요 문서

P1867

Sigma-Aldrich

Plasmin from human plasma

lyophilized powder, ≥2.0 units/mg protein

동의어(들):

Fibrinolysin

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About This Item

CAS Number:
EC 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
eCl@ss:
42030239
NACRES:
NA.54

양식

lyophilized powder

Quality Level

특이 활성도

≥2.0 units/mg protein

불순물

HIV, Hepatitis B and Hepatitis C, tested negative

UniProt 수납 번호

응용 분야

diagnostic assay manufacturing

저장 온도

−20°C

유전자 정보

human ... PLG(5340)

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일반 설명

Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes.

애플리케이션

A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin.

생화학적/생리학적 작용

It is inhibited by α 2-antiplasmin and its interaction with fibrin is blocked. It digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells.
Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products.
pH Optimum: 8.5
pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity
Temperature Optimum: 37 °C with rapid inactivation at 56 °C

포장

Package size based on protein content

단위 정의

One unit will produce one μmole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C.

물리적 형태

Lyophilized powder containing sodium phosphate and Trehalose.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

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문서 라이브러리 방문

Silke Haerteis et al.
The Journal of general physiology, 140(4), 375-389 (2012-09-12)
Proteolytic activation of the epithelial sodium channel (ENaC) involves cleavage of its γ subunit in a critical region targeted by several proteases. Our aim was to identify cleavage sites in this region that are functionally important for activation of human
Tatiana Syrovets et al.
Journal of leukocyte biology, 92(3), 509-519 (2012-05-09)
The serine protease plasmin generated from its zymogen plasminogen is best known for its function as a key enzyme of the fibrinolytic cascade. However, beyond fibrinolysis, plasmin has a number of crucial functions in a variety of processes, including inflammation.
S Müllertz et al.
The Biochemical journal, 159(3), 545-553 (1976-12-01)
A complex between plasmin and an inhibitor was isolated by affinity chromatography from urokinase-activated human plasma. The complex did not react with antibodies against any of the known proteinase inhibitors in plasma. A rabbit antiserum against the complex was produced.
On the mechanism of the reaction between human alpha 2-antiplasmin and plasmin.
B Wiman et al.
The Journal of biological chemistry, 254(18), 9291-9297 (1979-09-25)
Shu He et al.
British journal of haematology, 160(6), 806-816 (2013-01-31)
To assess whether Haemocomplettan(®) (fibrinogen concentrate) or Fibrogammin(®) (Factor XIII concentrate) can be used to manage bleeding complications of antithrombotic treatment, we examined a normal plasma pool spiked with AR-H067637 (thrombin inhibitor) or rivaroxaban (activated factor X-inhibitor), to which one

프로토콜

Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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