P0074

Anti-PERK (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): Anti-E2AK3, Anti-EIF2AK3, Anti-HsPEK, Anti-PEK, Anti-PRKR-like endoplasmic reticulum kinase, Anti-Pancreatic eIF2α kinase, Anti-WRS
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~150 kDa
• 종 반응성: human (predicted), mouse, rat
• 기술: western blot: 1:500-1:1,000 using whole cell lysate of HEK-293T expressing PERK cells
• UniProt 수납 번호: Q9NZJ5
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EIF2AK3(9451) , Eif2ak3(13666) ; mouse ... Eif2ak3(13666) ; rat ... Eif2ak3(29702)

설명

일반 설명

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) belongs to a family of eukaryotic initiation factor 2 α (eIF2α) kinases. It is a transmembrane kinase that is highly expressed in the pancreas. PERK contains two main domains: a kinase domain and an N-terminal domain like inositol- requiring enzyme-1 (IRE1), a protein involved in the unfolded protein response (UPR).

특이성

Anti-PERK C-terminal specifically recognizes PERK.

면역원

synthetic peptide corresponding to amino acids 1078-1092 of mouse PERK, conjugated to KLH. This sequence is identical in rat and differs by one amino acid in human.

애플리케이션

Anti-PERK (C-terminal) antibody produced in rabbit has been used for western blotting and ERK (extracellular-signal-regulated kinase) phosphorylation assays.

생화학적/생리학적 작용

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an endoplasmic reticulum (ER) stress sensor which phosphorylates eukaryotic initiation factor 2 α (eIF2α), thereby reducing the translation of most messenger RNAs (mRNAs). In mice models of frontotemporal dementia, the suppression of translation in a PERK-eIF2α-dependent manner plays a key role in neuronal loss. PERK confers protection to heart against pressure overload-induced congestive heart failure. Mutations in this gene are linked with Wolcott-Rallison syndrome (WRS) which is characterized by permanent neonatal diabetes, exocrine-deficient pancreas, growth retardation and osteopenia.

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) responds to distinct cellular stress signals.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)