추천 제품
제품명
Phosphatase Inhibitor Cocktail 3, DMSO solution
양식
DMSO solution
Quality Level
solubility
water: soluble
배송 상태
wet ice
저장 온도
2-8°C
애플리케이션
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.
생화학적/생리학적 작용
This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.
주의사항
As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.
제조 메모
This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
188.6 °F
Flash Point (°C)
87 °C
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