MSP13

Membrane Scaffold Protein 1D1 BTN

recombinant, expressed in E. coli, Biotin labeled

• 동의어(들): 1D1 BTN Protein, Membrane Protein Scaffold, Scaffold Protein 1D1 BTN
• UNSPSC 코드: 12352200
• NACRES: NA.26

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥90% (SDS-GE)
• 양식: buffered aqueous solution
• 분자량: 26,900.1 Da by calculation (without biotin)
• solubility: water: soluble
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Research area: Cell Struc

The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The MSP1D1 BTN variant of MSP1D1 features an enzymatically biotinylated additional 20-amino acid sequence at the C-terminus of MSP1D1.
Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1)

애플리케이션

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

생화학적/생리학적 작용

Scaffold proteins are vital for imparting specificity to the mitogen-activated protein kinase (MAPK) pathway, which is necessary for normal cellular functions and development. They assist in regulating the localization of MAPK components, enabling precise modulation of cellular responses while preserving overall MAPK activity.

법적 정보

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable