추천 제품
Quality Level
무균
0.2 μm filtered
양식
lyophilized powder
특이 활성도
≥4000 units/mg protein (biuret)
분자량
23 kDa
배송 상태
wet ice
저장 온도
−20°C
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일반 설명
Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.
애플리케이션
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
생화학적/생리학적 작용
Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
단위 정의
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
물리적 형태
Lyophilized powder containing Ficoll and sodium succinate buffer salts
제조 메모
Prepared from M 9901
기타 정보
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
John M Pfeffer et al.
Journal of bacteriology, 188(3), 902-908 (2006-01-24)
The O acetylation of peptidoglycan occurs specifically at the C-6 hydroxyl group of muramoyl residues. Using a combination of high-performance liquid chromatography-based organic acid analysis and carbohydrate analysis by high-pH anion-exchange chromatography, we determined that strains of Entercoccus durans, E.
Carli B Jones et al.
Frontiers in genetics, 13, 799615-799615 (2022-04-19)
Short read 16 S rRNA amplicon sequencing is a common technique used in microbiome research. However, inaccuracies in estimated bacterial community composition can occur due to amplification bias of the targeted hypervariable region. A potential solution is to sequence and assess
A rapid and efficient method of lysis of Listeria and other gram-positive bacteria using mutanolysin
Fliss, I., et al.
Biotechniques, 11, 456-457 (1991)
Tom Vanhoutte et al.
FEMS microbiology ecology, 48(3), 437-446 (2004-06-01)
According to the current insights, the predominant bacterial community in human feces is considered to be stable and unique for each individual over a prolonged period of time. In this study, the temporal stability of both the predominant population and
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal
프로토콜
This procedure may be used for Mutanolysin products.
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