M4071

MINK1 (1-320), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): B55, Hmink, MAP4K6, MGC21111, YSK2, ZC3, hMINKbeta
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 재조합: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• 제품 라인: PRECISIO^® Kinase
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 특이 활성도: 16-22 nmol/min·mg
• 분자량: ~61 kDa
• UniProt 수납 번호: Q8N4C8
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... MINK1(50488)

설명

일반 설명

Misshapen/NIKs-related kinase 1 (MINK1), also known as hMNIKβ or MINK is mapped to human chromosome 17p13.1. The gene codes for serine/threonine kinase, which is a member of the germinal center family of kinases (GCK) that are homologous to the yeast sterile 20 (Ste20) kinase family.

생화학적/생리학적 작용

Misshapen/NIKs-related kinase 1 (MINK1) plays a vital role in modulating various cellular process such as actin cytoskeleton rearrangement, cell matrix adhesion, cell-cell adhesion, cell morphology, and survival. It is also implicated in the cell motility and cell invasion. Kinase inactive mutant of MINK1 increased cell spreading, actin stress fiber formation, adhesion to extracellular matrix and inhibited cell motility and invasion. Thus, alteration in the MINK1 activity inhibits tumor development. MINK is activated after Ras induction via a method involving reactive oxygen species (ROS). This activated MINK triggers the activity of p38 stress-activated kinase via MAP3K (Mitogen-Activated Protein Kinase Kinase Kinase) 5/8 and MKK (mitogen-activated protein kinase kinase) 3/6, which leads to p53-independent induction of cyclin-dependent kinase inhibitor, p21^WAF1/CIP1 and permanent cell cycle arrest. MINK1 has an essential role in abscission, which is a final stage of cytokinesis.

물리적 형태

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)