LIG1

DNA Ligation Kit

• UNSPSC 코드: 12352204
• NACRES: NA.53

속성

• Grade: Molecular Biology
• Quality Level: 200
• 사용: 1 kit sufficient for 150 ligation reactions
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions. Sticky-end ligations are more efficient than blunt-end ligations; these can be facilitated with the addition of PEG.

애플리케이션

Suitable for:

• Joining fragments of DNA into a cloning vector
• Mutagenesis
• Gene anyalysis and structure-function relationships

성분

Sufficient for 150 reactions:

• 300uL 10X Ligation Buffer (D2176) in 250 mM Tris-HCl (pH 7.8) 100 mM MgCl2, and 10 mM dithiothreitol
• 3 x 100 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM dithiothreitol
• 3 x 100 uL 10 mM ATP ( A3702)
• 50 uL Control pBR322 DNA, HAE III Digest (D9430) 0.5 ug/ul in 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA
• 1.5 mL 24% (w/v) PEG Solution, (P 2454)
• 1.5 mL Molecular Biology Grade Water (W4502)

원리

One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319
• 예방조치 성명서: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable