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Merck
모든 사진(1)

주요 문서

L4516

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O127:B8

BioXtra, suitable for cell culture, γ-irradiated

동의어(들):

LPS

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352211
NACRES:
NA.75

생물학적 소스

Escherichia coli (O127:B8)

Quality Level

무균

γ-irradiated

제품 라인

BioXtra

양식

lyophilized powder

정제법

gel-filtration chromatography

기술

cell culture | mammalian: suitable

불순물

<1% Protein (Lowry)

solubility

H2O: 5 mg/mL, slightly hazy

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli 0127:B8 has been used:
  • to study the LPS-induced macrophage survival in mice
  • to elicit the secretion of cytokines by cattle PBMC
  • to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment
  • to perform autophagy assay in BV2 and BV2-LC3 cells
  • for stimulation of human monocytes, macrophages and mouse microglia cells
  • to induce pentraxin-3 expression in 293T cells

생화학적/생리학적 작용

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens.

재구성

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

기타 정보

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

픽토그램

Skull and crossbones

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

W A Jensen et al.
Journal of virology, 66(7), 4427-4433 (1992-07-01)
The in vitro expression of bovine leukemia virus (BLV) in short-term cultured bovine peripheral blood mononuclear cells (PBMC) is associated with increased spontaneous lymphocyte blastogenesis. The purpose of this study was to determine whether intracellular pathways responsible for antigen- or
Debby Kruijsen et al.
Vaccine, 29(15), 2730-2741 (2011-02-15)
Inactivated respiratory syncytial virus (RSV) vaccines tend to predispose for immune mediated enhanced disease, characterized by Th2 responses and airway hypersensitivity reactions. We show in a C57BL/6 mouse model that the early innate response elicited by the challenge virus (RSV
A Charlotte M T de Wolf et al.
PloS one, 12(6), e0179942-e0179942 (2017-06-29)
Regulatory T cells (Treg) function in the prevention of excessive inflammation and maintenance of immunological homeostasis. However, these cells may also interfere with resolution of infections or with immune reactions following vaccination. Effects of Treg on vaccine responses are nowadays
Hye-Eun Han et al.
Biomolecules & therapeutics, 21(1), 21-28 (2013-09-07)
Microglia play a role in maintaining and resolving brain tissue homeostasis. In pathological conditions, microglia release pro-inflammatory cytokines and cytotoxic factors, which aggravate the progression of neurodegenerative diseases. Autophagy pathway might be involved in the production of pro-inflammatory cytokines and
Hua Liu et al.
International journal of clinical and experimental pathology, 6(4), 660-668 (2013-04-11)
The aim of the present study was to explore the role of receptor tyrosine kinases (RTKs) in the regulation of expression of PTX-3, a protector in atherosclerosis. Human monocytic U937 cells were infected with a shRNA lentiviral vector library targeting

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