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Merck
모든 사진(1)

주요 문서

H7411

Sigma-Aldrich

Anti-HA−FITC antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

동의어(들):

Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.56

생물학적 소스

mouse

Quality Level

결합

FITC conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

HA-7, monoclonal

양식

buffered aqueous solution

저장 조건

protect from light

농도

1 mg/mL

기술

immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.

배송 상태

wet ice

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

특이성

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

면역원

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

애플리케이션

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:
  • in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
  • in western blotting detection of spindle-associated proteins in Trypanosoma brucei
  • in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

생화학적/생리학적 작용

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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