Horse serum displays homogeneity between fetal bovine serum and fetal calf serum .It has low levels of polyamine oxidase.

Horse serum is pooled from a normal donor herd.

Horse serum has been used as a supplement in minimum essential medium for cerebrocortical neuronal−glial cell cultures and RPMI-40 medium for PC12 cells derived from rat adrenal medulla pheochromocytoma. It has also been used in immunohistochemistry of Florida manatee brain sections.

Normal horse serum may be used as a blocking agent and controls in immunoassays. For immunohistochemistry and immunocytochemistry, 10% horse serum was used as blocking agent in various Studies.
Horse serum was used in immunostaining for blocking non-specific antibodies binding.

The horse serum is an alternative to bovine serum for growth factors and hormones for cell culture studies. Horse serum stimulates fibroblast differentiation and extracellular matrix (ECM) and cytokines synthesis.

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