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Merck
모든 사진(1)

주요 문서

GS71

Sigma-Aldrich

GST K1-1, Recombinant Human

동의어(들):

GST, GST 13-13, GST13, GST13-13, GSTK1-1, glutathione S-transferase kappa, hGSTK1

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.26

생물학적 소스

human

Quality Level

재조합

expressed in E. coli

분석

>95% (SDS-PAGE)

양식

frozen liquid

특이 활성도

12.22 units/mg protein

분자량

26 kDa

농도

3.17 mg/mL

저장 온도

−70°C

유전자 정보

human ... GSTK1(373156)

일반 설명

using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.

생화학적/생리학적 작용

Glutathione S-transferase kappa 1 (GSTK1) is an enzyme that in humans is encoded by the GSTK1 gene. Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

저장 및 안정성

The enzyme should be used by the end-user customer within 1 year of receipt.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

E V Kalinina et al.
Bulletin of experimental biology and medicine, 154(1), 64-67 (2013-01-19)
We studied the expression of genes encoding glutathione-S-transferase isoforms GSTP1-1, GSTA4-4, and GSTK1-1 during the development of the resistance of human erythroleukemia (K562), mammary adenocarcinoma (MCF-7) and ovary adenocarcinoma (SKOV-3) cells to cisplatin (CDDP). It was found that drug resistance
Anna Robinson et al.
The Biochemical journal, 379(Pt 3), 541-552 (2004-01-08)
The Kappa class of GSTs (glutathione transferases) comprises soluble enzymes originally isolated from the mitochondrial matrix of rats. We have characterized a Kappa class cDNA from human breast. The cDNA is derived from a single gene comprising eight exons and
Jie Li et al.
Protein science : a publication of the Protein Society, 14(9), 2361-2369 (2005-08-06)
Glutathione transferases (GSTs) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic
Fabrice Morel et al.
The Journal of biological chemistry, 279(16), 16246-16253 (2004-01-27)
Kappa class glutathione S-transferase (GST) cDNA sequences have been identified in rat, mouse, and human. In the present study, we determined the structure and chromosomal location of the human GST Kappa 1 (hGSTK1) gene, characterized the protein, and demonstrated its

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