G6916

Anti-GADD153 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-CHOP-10
• MDL number: MFCD03455044
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen 29 kDa
• 종 반응성: human
• 기술: immunocytochemistry: 5-10 μg/mL using Hela human cell; microarray: suitable; western blot (chemiluminescent): 1:200-1:400 using whole cell extract of HEK-293 over-expressing GADD 153.
• UniProt 수납 번호: P35638
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... DDIT3(1649) ; rat ... Ddit3(29467)

설명

일반 설명

Growth arrest and DNA damage inducible protein 153, C/EBP homology protein (GADD153/CHOP10) is a basic region leucine zipper transcription factor, devoid of DNA binding site. Its apparent molecular mass is 29 kDa (or 19 kDa calculated).

면역원

peptide corresponding to human GADD153/CHOP10 sequence (amino acids 153-169) with N-terminal lysine added, conjugated to keyhole limpet hemocyanin (KLH). The corresponding sequence in mouse and rat differs by 2 amino acids.

애플리케이션

Anti-GADD153 antibody produced in rabbit is suitable as a primary antibody in western blot analysis of:

• nuclear and cytoplasmic fractions extracted from rat insulinoma (INS) cells to study CHOP Expression in response to ER stress
• whole cell lysates prepared from ER stress induced rat pancreatic tissues
• whole cell lysates prepared from ER stress induced pancreatic insulin-producing cells

It is suitable for immunocytochemistry at a working concentration of 5-10μg/mL using Hela human cell and microarray. It is also suitable for western blotting at a working dilution of 1:200-1:400 using whole cell extract of HEK-293 over-expressing GADD 153.

생화학적/생리학적 작용

GADD153/CHOP10 (Growth Arrest and DNA Damage Inducible Protein 153, C/EBP Homology Protein) is a leucine zipper transcription factor that lacks a DNA binding site. The protein homodimerizes with members of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors during adipogenesis. Many signals induce the expression of CHOP10. Some of these signals include endoplasmic reticulum stress, hyperosmosis, growth arrest, DNA damage, calcium onophore, nutrient deprivation, hypoxia and hyperoxia. GADD153 heterodimerizes with C/EBPb and inhibits its binding to DNA sequences. Expresssion of this gene induces growth arrest in fibroblasts, inhibits adipocyte differentiation, and is associated with apoptosis in vitro. GADD153 binds to several nuclear proteins as well as the FTE/S3a ribosomal protein and enhances erythroid differentiation. It negatively regulates ATF3, a stress-inducible transcriptional repressor.

Growth arrest and DNA damage inducible protein 153, C/EBP homology protein (GADD153/CHOP10) is also involved in apoptosis in lungs of mice exposed to hyperoxia.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable