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Merck
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G1041

Sigma-Aldrich

EZBlue™ Gel Staining Reagent

동의어(들):

protein gel stain, protein stain

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About This Item

UNSPSC 코드:
41105300
NACRES:
NA.32

기술

protein staining: suitable

Quality Level

200

저장 온도

2-8°C

관련 카테고리

일반 설명

Convenient, sensitive, and safe, EZBlue™ Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

애플리케이션

EZBlue™ Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

특징 및 장점

  • Premixed solution eliminates the time and effort required to prepare the stain
  • Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)
  • Rapid reaction significantly reduces the amount of time required to stain and rinse
  • No solvent waste so you save time and money by eliminating hazardous material disposal

기타 정보

A ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.

법적 정보

EZBlue is a trademark of Sigma-Aldrich Co. LLC

픽토그램

Health hazardCorrosion
GHS08,GHS05

신호어

Warning

유해 및 위험 성명서

H290,H315,H319,H371

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

표적 기관

Eyes,Central nervous system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCC3649
SLBZ0323
SLBX5633
SLBW2004
SLBS5452

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문서

Preparing Cell Lysates

This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot.

Western Blot Analysis of Immunoprecipitation (IP-Western)

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Sample Preparation & Gel Electrophoresis Troubleshooting

The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS

The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.

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