추천 제품
양식
liquid
Quality Level
분석물 화학적 분류
proteins (Immunoglobulins of various mammalian species)
기술
affinity chromatography: suitable
immunoprecipitation (IP): suitable
용량
≥8 mg/mL, gel binding capacity (rabbit IgG)
저장 온도
−20°C
일반 설명
EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
애플리케이션
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
특징 및 장점
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
물리적 형태
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%
법적 정보
EZview is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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