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D3937

Sigma-Aldrich

DirectLoad™ 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

동의어(들):

1 kb marker, 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, 1kb marker for gel electrophoresis, DNA ladder, DNA marker, DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, ready to use DNA ladder, ready to use DNA marker

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About This Item

UNSPSC 코드:
41105335
NACRES:
NA.25

Quality Level

200

양식

liquid

사용

100 uses

적합성

suitable for electrophoresis (DNA)

저장 온도

−20°C

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관련 카테고리

일반 설명

Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

애플리케이션

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

특징 및 장점

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

성분

Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

기타 정보

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.

법적 정보

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
SLCJ8430
SLCJ6694
SLCJ1324
SLCG8770
SLCD9298

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문서

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

프로토콜

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

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