MDA-MB-231 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92020424, human breast, Epithelial

• 동의어(들): MD Anderson-Metastatic Breast-231 Cells, MDA-MB231 Cells, MDA231-BRE Cells, MDAMB231 Cells
• UNSPSC 코드: 41106514
• eCl@ss: 32190102

속성

• 생물학적 소스: human breast
• 포장: tube of 5 μg 92020424-DNA-5UG; pkg of vial of cells 92020424-1VL
• 성장 모드: Adherent
• 핵형: Modal no.′s 62 and 64, near triploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian breast adenocarcinoma

세포주 설명

Isolated from pleural effusions of a breast cancer patient.

애플리케이션

MDA-MB-231 has been used to study:

• the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies
• the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system
• the transendothelial migration rates of the cells under different shear stress levels

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18

배양 배지

L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm² using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO₂ is not required.

기타 정보

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면책조항

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.