C7438

Chaperonin 10 from Escherichia coli

≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• 동의어(들): GroES
• MDL number: MFCD00163256
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: Escherichia coli
• Quality Level: 200
• 재조합: expressed in E. coli overproducing strain
• 분석: ≥95.0% (SDS-PAGE)
• 양식: lyophilized powder
• 기술: activity assay: suitable
• UniProt 수납 번호: P61604
• 저장 온도: 2-8°C
• 유전자 정보: human ... HSPE1(3336)

설명

생화학적/생리학적 작용

Chaperonin60 (GroEL) and chaperonin10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

포장

Package size based on protein content.

물리적 형태

Lyophilized powder containing Tris buffer salts, potassium chloride, dithiothreitol and trehalose as stabilizer.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)