추천 제품
Grade
Proteomics Grade
Quality Level
양식
salt-free, lyophilized powder
분자량
25 kDa
적합성
suitable for protein sequencing
UniProt 수납 번호
저장 온도
2-8°C
유전자 정보
cow ... CTRB1(618826)
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관련 카테고리
일반 설명
α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.
Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.
애플리케이션
α-Chymotrypsin from bovine pancreas is used for the following applications:
- Protein Identification by mass spectrometry (MS)
- The isolation and characterization of myosin heavy chains
- Toxin preparation
- The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE
생화학적/생리학적 작용
α-Chymotrypsin from bovine pancreas is stabilized by Ca2+ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu2+ and Hg.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
단위 정의
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
기타 정보
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
기질
제품 번호
설명
가격
저해제
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
개인 보호 장비
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
이미 열람한 고객
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Rausch C, et al.
BioChemistry: An Indian Journal, 9(6), 213-220 (2015)
L M Simon et al.
Biochemical and biophysical research communications, 280(5), 1367-1371 (2001-02-13)
The effects of different concentrations (20-95%) of organic solvents (ethanol, 1,4-dioxane and acetonitrile) were studied on alpha-chymotrypsin and trypsin from bovine pancreas. The changes in secondary structure were followed by CD measurements, and the apparent Michaelis constants (KMapp) and the
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문서
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
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