C0979

Anti-Catalase antibody ,Mouse monoclonal

clone CAT-505, purified from hybridoma cell culture

• 동의어(들): Anti-CAT
• MDL number: MFCD00802760
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: CAT-505, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen 60 kDa
• 종 반응성: human, mouse, bovine, rat
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells
• 동형: IgG1
• UniProt 수납 번호: P04040
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CAT(847) ; mouse ... Cat(12359) ; rat ... Cat(24248)

설명

일반 설명

Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa.

면역원

human erythrocyte catalase

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (5 papers)

Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following:

• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Microarray
• Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells
• Immunofluorescence

생화학적/생리학적 작용

Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide

제조 메모

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable