추천 제품
애플리케이션
Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.
특징 및 장점
- Simple, sensitive colorimetric assay for proteins
- Two stable reagents are used for the working solution
- Faster and easier than the Lowry protein assay
- Compatible with many detergents both ionic and nonionic
- Less variation between different proteins than the Bradford dye-binding assay
- Adaptable for use in microwell plates
원리
Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.
키트 구성품 전용
제품 번호
설명
- Bicinchoninic Acid Solution 1 L
- 4%(w/v) CuSO4 · 5H2O Solution 25 mL
- Protein Standard Solution 5 x 1
애플리케이션
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Salivary proteome patterns of individuals exposed to High Altitude
Jain S, et al.
Archives of Oral Biology, 96, 104-112 (2018)
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TGFB1 modulates in vitro secretory activity and viability of equine luteal cells
Galvao A, et al.
Cytokine, 110, 316-327 (2018)
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Physiological genomics, 51(7), 290-301 (2019-05-28)
Using CRISPR-Cas9 technology, we created a 5-HT7 receptor global knockout (KO) rat, on a Sprague-Dawley background, for use in cardiovascular physiology studies focused on blood pressure regulation. A stable line carrying indels in exons 1 and 2 of the rat
관련 콘텐츠
A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.
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