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일반 설명
Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
애플리케이션
Casein Blocking Buffer 10x has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
특징 및 장점
- Better blocking performance than dried milk or BSA
- Easy-to-use concentrate
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent and colorimetric detection assays
제조 메모
Dilute 10x Blocking Buffer with molecular biology grade water before use.
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제품 번호
설명
가격
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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프로토콜
Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.
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