추천 제품
양식
lyophilized powder
Quality Level
특이 활성도
(chromatography purified)
≥4 units/mg protein
구성
Protein, 30-50% biuret
solubility
H2O: soluble 1.0 mg/mL, clear, colorless
저장 온도
−20°C
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관련 카테고리
애플리케이션
Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms. It has also been used as a Azocasein substrate for the determination of proteolytic activity.
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
생화학적/생리학적 작용
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
단위 정의
One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.
물리적 형태
Lyophilized powder containing mannitol and potassium phosphate buffer salts
제조 메모
Chromatographically purified
Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
H Umesh Hebbar et al.
Bioresource technology, 99(11), 4896-4902 (2007-10-30)
Reverse micellar systems of CTAB/isooctane/hexanol/butanol and AOT/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). The effect of forward as well as back extraction process
Rubab Mahmood et al.
Biochemistry. Biokhimiia, 72(3), 307-312 (2007-04-24)
Stem bromelain was covalently coupled to a thermosensitive polymer of N-isopropylacrylamide (p(NIPAm)) either through the amino groups of the enzyme (randomly coupled) or via the lone oligosaccharide chain (uniformly coupled). The enzyme coupled via the oligosaccharide chain exhibited better access
Hafeeza Khatoon et al.
Protein and peptide letters, 14(3), 233-236 (2007-03-10)
The lone oligosaccharide chain of stem bromelain was oxidized with periodic acid to generate aldehyde groups and the resulting oxidized enzyme coupled to amino-Sepharose in order to obtain an immobilized preparation with uniformly oriented enzyme. The immobilized bromelain exhibited high
Soghra Khatun Haq et al.
The international journal of biochemistry & cell biology, 37(2), 361-374 (2004-10-12)
The effect of salts and alcohols was examined on the partially folded intermediate (PFI) state of stem bromelain reported at low pH (Haq, Rasheedi, and Khan (2002) European Journal of Biochemistry 269, 47-52) by a combination of optical methods like
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