B0184

Bacteriorhodopsin from Halobacterium salinarum

native sequence, lyophilized powder

• 동의어(들): BR from H. salinarum, Bacterioopsin, Bacteriorhodopsin from Halobacterium halobium
• CAS Number: 53026-44-1
• MDL number: MFCD00130599
• UNSPSC 코드: 12352202
• NACRES: NA.56

속성

• 생물학적 소스: Halobacterium salinarium
• Quality Level: 100
• 양식: lyophilized powder
• 기술: ligand binding assay: suitable; mass spectrometry (MS): suitable
• UniProt 수납 번호: B0R5N9
• 저장 온도: 2-8°C
• 유전자 정보: Halobacterium salinarium ... OE_RS05715(5953595) , VNG_RS05715(144807)

설명

일반 설명

Bacteriorhodopsin (BR) is a covalent complex comprising bacterioopsin protein and retinal cofactor in the equimolar ratio. It corresponds to the molecular weight of 27kDa. BR belongs to the retinylidene class of proteins. It is a seven-membrane helical protein that acts as a photon-driven pump. BR can be used in studies of the folding and kenetics of β-helical proteins.

Bacteriorhodopsin is the prototypical "seven-helix" transmembrane protein (with seven α-helical domains), whose study led to advances in understanding G protein-coupled receptors (GPCRs). In Halobacteria, it acts as a light-harvesting protein, producing a proton gradient across the cell wall that is then used to drive biosynthetic processes.

애플리케이션

Bacteriorhodopsin from Halobacterium salinarum has been used:

• in generation of droplet lipid bilayer
• as a standard in quadrupole time-of-flight (QTOF) mass spectroscopy (MS)
• in the generation of protein-detergent complex and micelles for dynamic light scattering studies

Bacteriorhodopsin is of interest in the development of artificial retinas, optical associative processors, and three-dimensional memory storage devices.

생화학적/생리학적 작용

A transmembrane retinylidine protein that functions as a proton pump driven by light energy in Holobacterium.

Bacteriorhodopsin (BR) from Halobacterium salinarum acts as a proton-driven pump. BR can be used in studies of the folding and kinetics of α-helical proteins. It is thermally stable and exhibits high photoelectric and photochemical efficiency. BR exists as trimer in a hexagonal lattice. Its photocycle intermediates are exploited in bioelectronics majorly in photoelectric and photochemical applications.

제조 메모

Aqueous suspensions may be sonicated to achieve the desired homogeneity and may be stored for a short time at a temperature of 2-8 °C or at a temperature of -20 °C without time limitation.

Wild-type bacteriorhodopsin is isolated from Halobacterium salinarum strain S9 as purple membranes.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)