추천 제품
생물학적 소스
egg white
Quality Level
결합
agarose conjugate
양식
aqueous glycerol suspension
Matrix
cross-linked 6% beaded agarose
기질 활성
epichlorohydrin
기질 부착
amino
기질 스페이서
15 atoms
저장 온도
−20°C
일반 설명
Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.
애플리케이션
Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
- in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
- in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
- in avidin bead assay for purification of labelled biotin
- for capture of glycoproteins from murine cornea samples
생화학적/생리학적 작용
Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.
단위 정의
One unit will bind 1.0 μg of d-biotin.
물리적 형태
Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
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관련 콘텐츠
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
친화성, GST 풀다운, TAP, 공동 면역 침전법을 활용한 풀다운 분석을 통해 in vitro 단백질-단백질 상호작용을 분석합니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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