A5713
Monoclonal Anti-polyHistidine–Agarose antibody produced in mouse
clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins
동의어(들):
Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
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모든 사진(1)
About This Item
UNSPSC 코드:
12352203
NACRES:
NA.56
추천 제품
생물학적 소스
mouse
Quality Level
결합
agarose conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
HIS-1, monoclonal
양식
PBS suspension
동형
IgG2a
용량
15-30 nmol/mL binding capacity
적합성
suitable for purification of HIS tagged recombinant proteins
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
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일반 설명
This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.
특이성
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
면역원
recombinant polyHistidine tagged fusion protein.
애플리케이션
Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
물리적 형태
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
제조 메모
Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.
법적 정보
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Eric A Davidson et al.
Current protocols in molecular biology, Chapter 24, Unit 24-Unit 24 (2009-07-04)
This unit describes a protocol for the directed evolution of proteins utilizing in vitro compartmentalization. This method uses a large number of independent in vitro transcription and translation (IVTT) reactions in water droplets suspended in an oil emulsion to enable
Dong Meng et al.
The Journal of biological chemistry, 284(17), 11425-11435 (2009-01-21)
betaArrestin is a multifunctional signal scaffold protein. Using SPOT immobilized peptide arrays, coupled with scanning alanine substitution and mutagenesis, we show that the MAPK kinase, MEK1, interacts directly with betaarrestin1. Asp(26) and Asp(29) in the N-terminal domain of betaarrestin1 are
Ryan T Cameron et al.
Molecular and cellular neurosciences, 61, 46-55 (2014-05-27)
Up-regulation of Hsp20 protein levels in response to amyloid fibril formation is considered a key protective response against the onset of Alzheimer's disease (AD). Indeed, the physical interaction between Hsp20 and Aβ is known to prevent Aβ oligomerisation and protects
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