추천 제품
생물학적 소스
bovine
Quality Level
양식
powder
기원
USA origin
기술
affinity chromatography: suitable
fractionation: suitable
불순물
≤10% water (Karl Fischer)
UniProt 수납 번호
저장 온도
2-8°C
유전자 정보
bovine ... ALB(280717)
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애플리케이션
Albumin methylated from bovine serum (mBSA) has been used as a coating for immunoplates in enzyme-linked immunosorbent assay (ELISA) of anti-dsDNA IgG antibodies. It has also been used to induce arthritis in mice models.
As an affinity ligand on diatomaceous earth support (MAK chromatography), a medium for fractionation of different forms of DNA and for separating aminoacyl-tRNAs. Different modes of binding of double-stranded, denatured, and replicating forms of DNA account for the efficacy of the method.
생화학적/생리학적 작용
Albumin is a major circulating component of the plasma and is a highly soluble multifunctional protein. It plays a role in several physiological and pharmacological processes. Albumin binds to metals, cholesterol, hormones, bile pigments, drugs, and fatty acids to facilitate their transport. It regulates osmotic pressure and distributes fluids between various compartments.
제조 메모
Methyl ester of albumin prepared per Mandell, J.D. and Hershey, A.D., Anal. Biochem., 1, 66 (1960).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
Fractionation of nucleic acids with the methylated albumin column.
N SUEOKA et al.
Journal of molecular biology, 4, 161-172 (1962-03-01)
T Hofstaetter et al.
Biochimica et biophysica acta, 518(1), 53-60 (1978-03-29)
The binding of methylated albumin to DNA, the basis of the chromatography on columns of kieselguhr coated with methylated albumin (MAK chromatography), was investigated. Scatchard plots revealed only one mode of interaction with fully double-stranded DNA. The complexes should be
Kasper Almholt et al.
Journal of immunology (Baltimore, Md. : 1950), 200(3), 957-965 (2017-12-29)
Genetic absence of the urokinase-type plasminogen activator (uPA) reduces arthritis progression in the collagen-induced arthritis (CIA) mouse model to an extent just shy of disease abrogation, but this remarkable observation has not been translated into therapeutic intervention. Our aim was
Fractionation of amino acyl-acceptor RNA on a methylated albumin column.
N SUEOKA et al.
Proceedings of the National Academy of Sciences of the United States of America, 48, 1454-1461 (1962-08-01)
Mohammad Haque et al.
Scientific reports, 6, 20588-20588 (2016-02-06)
Pluripotent stem cells (PSCs) have the potential to produce almost all of the cells in the body, including regulatory T cells (Tregs). However, the exact conditions required for the development of antigen (Ag)-specific Tregs from PSCs (i.e., PSC-Tregs) are not
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