60700

Nuclear Fast Red

• 동의어(들): 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt
• 실험식(Hill 표기법): C₁₄H₈NNaO₇S
• CAS Number: 6409-77-4
• Molecular Weight: 357.27
• 색상 지수 번호: 60760
• EC Number: 229-088-0
• MDL number: MFCD00078493
• UNSPSC 코드: 12171500
• PubChem Substance ID: 57651779
• NACRES: NA.47

속성

• 양식: powder
• Quality Level: 100
• 기술: microbe id | staining: suitable
• solubility: methanol: water (1:1): 0.01 g/10 mL, red
• εmax: ≥275 at 550-560 nm in DMSO; ≥300 at 516-526 nm in DMSO
• 적합성: suitable for microscopy
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: [Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
• InChI: 1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
• InChI key: IFSXZLJQEKGQAF-UHFFFAOYSA-M

설명

일반 설명

Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt. It is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a dry staining dye that makes target structures (by fixing, wherenecessary embedding, staining with the above nuclear fast red solution, counterstaining, mounting) in human-histological specimen materials, for examplehistological sections of e. g. the kidney, evaluable for diagnostic purposes.

애플리케이션

Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:

• staining sperm and spermatids in semen
• identifying areas for PCR analysis in paraffin-embedded tissues
• nuclear counterstaining following immunostaining and silver staining

Used as a biological stain

생화학적/생리학적 작용

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

원리

Nuclear fast red can be used for simple nuclear staining. It is an acidic dyebelonging to the group of anthraquinone dyes. Aluminum sulfate, functioning as a mordant, is added to form a color lake that enables red nuclear staining. Nuclear fast red-aluminum sulfate is used for counterstaining in histochemicalreactions, e. g. in Berlin blue staining for detection of iron or in silver staining. Nuclear fast red for microscopy Certistain^® can also be used fornuclear staining with alcian blue.

법적 정보

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves