54457

HEPES

≥99.5% (titration), powder, for molecular biology, BioUltra

• 동의어(들): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• 실험식(Hill 표기법): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• UNSPSC 코드: 12161700
• PubChem Substance ID: 57651473
• NACRES: NA.25

속성

• 제품명: HEPES, BioUltra, Molecular Biology, ≥99.5% (T)
• Grade: Molecular Biology
• Quality Level: 200
• 제품 라인: BioUltra
• 분석: ≥99.5% (T)
• 양식: powder
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• 무기 잔류물: ≤0.1% (as SO₄)
• 손실: ≤0.5% loss on drying, 110 °C
• pH: 5.0-6.5 (25 °C, 1 M in H₂O)
• 유용한 pH 범위: 6.8-8.2
• pKa(25 °C): 7.5
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• 음이온 미량물: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV 흡수: λ: 260 nm A_max: 0.045; λ: 280 nm A_max: 0.035
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

일반 설명

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

애플리케이션

HEPES has been used as a component of:

• DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
• RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
• Recapture solution to re-capture RNA exosomes from the glycerol gradient
• ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
• 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

기타 정보

3′-Terminal labeling of RNA with T4 RNA ligasecitation; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffercitation.

Easily compare specifications for HEPES products with the HEPES specification table.

3′-Terminal labeling of RNA with T₄ RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)